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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/10/2002 |
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Data da última atualização: |
01/10/2002 |
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Autoria: |
SCHUSTER, I.; ABDELNOOR, R. V.; MARIN, S. R. R.; CARVALHO, V. P.; KIIHL, R. A. S.; SILVA, J. F. V.; SEDIYAMA, C. S.; BARROS, E. G.; MOREIRA, M. A. |
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Título: |
Identification of a new major QTL associated with resistance to soybean cyst nematode (Heterodera glycines). |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Theoretical and Applied Genetics, New York, v. 102, n. 1, p. 91-96, 2001. |
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Idioma: |
Inglês |
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Conteúdo: |
Resistance of soyabean (Glycine max) to cyst nematode (SCN) (Heterodera glycines), one of the most destructive pathogens affecting soyabean, involves a complex genetic system. The identification of QTLs associated with SCN resistance may contribute to the understanding of such system. The objective of this work was to identify and map QTLs for resistance to SCN Race 14 with the aid of molecular markers. BC3F2:3 and F2:3 populations, both derived from an original cross between resistant soyabean cv. Hartwig and the susceptible line BR-92-31983 were screened for resistance to SCN Race 14. Four
microsatellite (Satt082, Sat_001, Satt574 and Satt301) and four RAPD markers (OPAA-11795, OPAE-08837, OPR-07548 and OPY-072030) were identified in the BC3F2:3 population using the bulked segregant analysis (BSA) technique. These markers were amplified in 183 F2:3 families and mapped to a locus that accounts for more than 40% of the resistance to SCN Race 14. Selection efficiency based on these markers was similar to that obtained with the conventional method. In the case of the microsatellite markers, which identify homozygous resistant genotypes, the efficiency was even higher. This new QTL has been mapped to the soyabean linkage group D2 and, in conjunction with other QTLs already identified for SCN resistance, will certainly contribute to understanding of the genetic basis of resistance of this important disease in soyabean. |
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Categoria do assunto: |
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Marc: |
LEADER 02096naa a2200217 a 4500
001 1463273
005 2002-10-01
008 2001 bl --- 0-- u #d
100 1 $aSCHUSTER, I.
245 $aIdentification of a new major QTL associated with resistance to soybean cyst nematode (Heterodera glycines).
260 $c2001
520 $aResistance of soyabean (Glycine max) to cyst nematode (SCN) (Heterodera glycines), one of the most destructive pathogens affecting soyabean, involves a complex genetic system. The identification of QTLs associated with SCN resistance may contribute to the understanding of such system. The objective of this work was to identify and map QTLs for resistance to SCN Race 14 with the aid of molecular markers. BC3F2:3 and F2:3 populations, both derived from an original cross between resistant soyabean cv. Hartwig and the susceptible line BR-92-31983 were screened for resistance to SCN Race 14. Four microsatellite (Satt082, Sat_001, Satt574 and Satt301) and four RAPD markers (OPAA-11795, OPAE-08837, OPR-07548 and OPY-072030) were identified in the BC3F2:3 population using the bulked segregant analysis (BSA) technique. These markers were amplified in 183 F2:3 families and mapped to a locus that accounts for more than 40% of the resistance to SCN Race 14. Selection efficiency based on these markers was similar to that obtained with the conventional method. In the case of the microsatellite markers, which identify homozygous resistant genotypes, the efficiency was even higher. This new QTL has been mapped to the soyabean linkage group D2 and, in conjunction with other QTLs already identified for SCN resistance, will certainly contribute to understanding of the genetic basis of resistance of this important disease in soyabean.
700 1 $aABDELNOOR, R. V.
700 1 $aMARIN, S. R. R.
700 1 $aCARVALHO, V. P.
700 1 $aKIIHL, R. A. S.
700 1 $aSILVA, J. F. V.
700 1 $aSEDIYAMA, C. S.
700 1 $aBARROS, E. G.
700 1 $aMOREIRA, M. A.
773 $tTheoretical and Applied Genetics, New York$gv. 102, n. 1, p. 91-96, 2001.
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